Fig. 7: Clonotypic features of ST3-reactive B cells. | Communications Biology

Fig. 7: Clonotypic features of ST3-reactive B cells.

From: Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to pneumococcal vaccination

Fig. 7

a UMAP colored by isotype of recovered BCR. b Isotypes recovered from B cell phenotypes. c UMAP colored by frequency of somatic mutation. d Frequency of somatic mutation in heavy chain sequences recovered from each phenotype. P-values are calculated with a two-sided Wilcoxon rank-sum test and are adjusted with Bonferroni correction. e Clonal sizes of ST3-reactive and ST3- B cells. Frequency of IGHV (f) and IGKV/IGLV (g) genes among ST3+ cells from vaccinated monkeys and all ST3- cells. Genes highlighted in red are statistically significantly enriched in the ST3+ fraction (p < 0.001, p values calculated using a two-sided chi-squared test and are adjusted using Bonferroni correction). h CDRH3 junction lengths of ST3-reactive cells using IGHV genes statistically associated with ST3-reactive cells. i IGKV/IGLV gene pairings lengths of ST3-reactive cells using IGHV genes statistically associated with ST3-reactive cells. j Logo plots of CDRH3 junctions of ST3-reactive clonotypes using IGHV4-173, IGHV4-80, and IGHV4-99. k Number of clonotypes with each V-gene recovered from each vaccinated and unvaccinated monkey.

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