Fig. 1: Cys supplementation increases antimicrobial resistance in E. coli.

A–I Respiration signal of E. coli wildtype W and three clinical isolates, GN02007, GN02094, and GN02148 in the absence and presence of 5 mM Cys with diverse antibiotics contained in phenotype microarray plates. A amoxicillin (0.03 mM), B bleomycin (0.092 mM), C capreomycin (0.046 mM), D cefazolin (0.693 mM), E ceftriaxone (0.004 mM), F cephalothin (0.049 mM), G cloxacillin (2.36 mM), H enoxacin (0.019 mM), I erythromycin (0.098 mM). In all conditions, respiration signals were high when Cys was supplemented. Results for the full antibiotics are summarized in Table S5. The experiments were performed in biological duplicate and shadow indicates the maximum and minimum value of each measurement.