Fig. 5: Sequence alignment of the upstream regions of CyuR in various microorganisms in the family of Enterobacteriaceae.
From: CyuR is a dual regulator for L-cysteine dependent antimicrobial resistance in Escherichia coli
A Sequence alignments of the upstream regions of cyuR in different microorganisms. Known SoxS binding motif and predicted CyuR-binding motif are colored in green and yellow, respectively. Asterisk indicates the commonality of corresponding bases. Each sequence starts with a feature ID that was given by the SEED database (https://www.theseed.org). B Sequence logos were generated based on the multiple alignments of CyuR-binding sites from multiple groups of Enterobacteria genomes (see Fig. S7) using the WebLogo tool (https://weblogo.berkeley.edu/logo.cgi). C The fluorescence polarization of CyuR and 10 nM fluorescently labeled 30 bp DNA fragment of the upstream sequence of the cyuR (see “Methods” section). This assay was performed in the presence and absence of 15 mM L-Cys with biological duplicate. x-axis and y-axis indicate CyuR concentration (μM) and fluorescence polarization, respectively. NC indicates the negative control which is HisR from R. gnavus (see “Methods” section).
