Fig. 2: ICVB-1042 binds and enters A549 cells via CD46. | Communications Biology

Fig. 2: ICVB-1042 binds and enters A549 cells via CD46.

From: Nonclinical characterization of ICVB-1042 as a selective oncolytic adenovirus for solid tumor treatment

Fig. 2

a Human plasma membrane proteins (6101 full-length proteins and 396 heterodimers) were screened in technical duplicate for binding to ICVB-1042 and Wt Ad5 using the Retrogenix™ Cell Microarray Technology. Subsequent confirmatory screening (n = 34 interactions) in technical duplicate revealed specific binding of ICVB-1042 to CD46 isoform D and CD46 isoform A while Wt Ad5 bound to CXADR (CAR) and glycoprotein 2 (GP2). Neither virus specifically bound to desmoglein 2 (DSG2). b–d YPet fluorescence on CD46+ A549 and CD46− A549 cells were assessed following incubation with ICVB-1042 or Ad5-YPet (24 h at MOI = 10) by flow cytometry. b Representative flow cytometry plots showing YPet fluorescence on CD46+ A549 and CD46− A549 cells. c Percentages of YPet+ cells. Bars represent means of technical duplicates. d Relative percentage of YPet+ cells within CD46− A549 cells, normalized to percentage of YPet+ cells within CD46+ A549 cells.

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