Fig. 1: Structural aberration of brain after chronic hypoxia.
From: Atoh1 mediated disturbance of neuronal maturation by perinatal hypoxia induces cognitive deficits
a 3D graphs of control and model mice in P11.5 brain by T2 scanning (A, anterior; L, left; D, dorsal). Bar graphs on the left show the total brain volume of control and model mice at P11.5 and P30 (control, n = 6; model, n = 6) (For statistics, see Table S3). Bar graphs on the right show the normalized total brain volume of control and model mice at P11.5 and P30 (control, n = 6; model, n = 6) (For statistics, see Table S4). b Staining of cerebellar lobule IV-V by antibodies against Ki67 and NeuN in mice at P11.5. The three right panels show a magnified view of dashed squares on the left. (EGL, external granular layer; ML, molecular layer; IGL, internal granular layer) Scale bars: 50 μm. (column on the left, objective 20x; three right columns, objective 40x). Bar graph above shows the ratio of Ki67 positive cells in EGL. Bar graph below shows the ratio of NeuN positive cells in EGL (control, n = 6; model, n = 6) (For statistics, see Table S5). c Staining of cerebellar lobule III by antibodies against Pax6 and NeuN in mice at P11.5. Scale bars: 50 μm. Objective 20x. White arrows indicate double positive cells. Bar graph above shows the number of Pax6 and NeuN positive cells in EGL (control, n = 6; model, n = 6). Bar graph below shows the number of Pax6 and NeuN positive cells in ML (control, n = 6; model, n = 6) (For statistics, see Table S6). d Staining of hippocampal CA1 by antibodies against SYP and NeuN in mice at P11.5. The three right panel shows a magnified view of dashed squares on the left. HPC, hippocampus. Scale bars: 50 μm (columns on the left, objective 20x); 20 μm (three right columns, objective 63x). Bar graph above shows mean fluorescence intensity (MFI) of SYP staining, data were presented as relative to control (control, n = 6; model, n = 6). Bar graph below shows density of SYP+ points on per neuron (control, n = 16; model, n = 16) (For statistics, see Table S7). e Protein fractions from brains of P11.5 mice were tested with TrkB, BDNF, SYP, and Sema4F. Left panels show representative bands of proteins mentioned above. β-actin was used as an internal control. Bar graphs on the right show percentage changes of proteins in model mice relative to control (control, n = 3; model, n = 3) (For statistics, see Table S8). All data were shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
