Fig. 1: Diagrams of the cytosine base editor and ebony-gRNA constructs.
From: Expanding the CRISPR base editing toolbox in Drosophila melanogaster
A Two promoters were used, a nos promoter for germline-specific expression and an actin promoter for ubiquitous base editor expression. Nos-5′ nos promoter, nos-3 nos terminator, p10-3′ p10 terminator, actin-5′ actin promoter, mini-w mini-white selectable marker, PhiC31 AttB recognition site for PhiC31-mediated integration. AmpR ampicillin resistance gene, ori bacterial origin of replication. B Diagram of the ebony-gRNA construct. dU6-1 and dU6-3 are both Drosophila U6 promoters for ubiquitous gRNA expression. C A schematic of the gRNAs used to target an ebony exon. The gRNA sequence is highlighted in gray, PAM (red) and the target bases (orange) for each gRNA are also highlighted.
