Fig. 3: Stop codon molecular analysis of editing by CBEs.
From: Expanding the CRISPR base editing toolbox in Drosophila melanogaster
A–D The percentage of reads with a stop codon compared to the predicted percentage of ebony flies from the Amp-Seq subset of the phenotyping assay. The stop codon percentage at either gRNA1/gRNA2 was determined from the Amp-Seq data (n = 2, the total number of flies used per cross is detailed in Supplementary Table 4, data is individual measurements (points)). The predicted ebony percentage was the percentage of flies from the Amp-Seq data subset that were phenotypically ebony in our experiments from Fig. 2. E Diagram of how maternal deposition of a CBE may increase the stop codon abundance above the theoretical editing threshold of 50%.
