Fig. 4: Frequency of different types of base substitution at the target C bases.
From: Expanding the CRISPR base editing toolbox in Drosophila melanogaster
A The gRNA sequences for gRNA1 and gRNA2 are detailed (black), with the target cytidines (orange) numbered and the PAM sequence in red (data presented as the mean frequency of reads with the specified substitution out of all reads harboring a non-C nucleotide at the target base; n = 2, the total number of flies used per cross is detailed in Supplementary Table 4). B–E The effect of different CBE transmission modes on base substitution frequency was assessed by examining the four transmission pathways.
