Fig. 8: The inhibition of MD-ACC pathway relieved mechanical pain and produced conditioned place preference (CPP) after nerve injury. | Communications Biology

Fig. 8: The inhibition of MD-ACC pathway relieved mechanical pain and produced conditioned place preference (CPP) after nerve injury.

From: Deconstruction the feedforward inhibition changes in the layer III of anterior cingulate cortex after peripheral nerve injury

Fig. 8: The inhibition of MD-ACC pathway relieved mechanical pain and produced conditioned place preference (CPP) after nerve injury.

A Upper: Diagram to show the experiment design of mCherry and hM4Di expression in MD and cannula implanted in ACC. Lower: The timeline of the experiment. B a–c: Representative images showed the expression of Retro-cre-GFP in ACC and the expression of mCherry and hM4Di in MD, scale bar: 200 μm. C Representative image showed the location of the cannula in ACC, scale bar: 500 μm. D Representative traveling traces of the mCherry and hM4Di mice in the CPP test. E The application of CNO to the hM4Di group induced CPP (Two-way ANOVA, Interaction, F(1, 16) = 5.599, P = 0.0309; Sidak’s multiple comparisons test, mCherry vs. hM4Di (Test): P = 0.0271; In hM4Di group, pre vs. test: P = 0.042. mCherry: n = 9, hM4Di: n = 9). F The application of CNO increased the hM4Di group mechanical pain threshold, not the mCherry group (Two-way ANOVA, Interaction, F(3, 36) = 5.54, P < 0.01; mCherry vs. hM4Di (CPN + CNO), P = 0.03; mCherry: n = 6, hM4Di: n = 8).

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