Fig. 7: Low-frequency SEMFs promoted osteogenic differentiation of BMSCs by regulating miR-34b-5p/STAC2 axis. | Communications Biology

Fig. 7: Low-frequency SEMFs promoted osteogenic differentiation of BMSCs by regulating miR-34b-5p/STAC2 axis.

From: Low frequency sinusoidal electromagnetic fields promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells by modulating miR-34b-5p/STAC2

Fig. 7

a ALP and ARS staining assays of BMSCs transfected with miR-34b-5p mimic or STAC2 siRNA under low-frequency SEMFs stimulation. b Semi-quantitative analyses of ALP and ARS staining intensities. The quantitative analyses of staining intensities were normalized based on changes in cell number resulting from the modulation of miR-34b-5p activity. c The mRNA expression levels of BMP2, ALP, RUNX2, and OCN after transfection of miR-34b-5p mimic and STAC2 siRNA under 7-day low-frequency SEMFs stimulation. d Westen blot results showing the osteogenic makers (β-catenin, Active-β-catenin, RUNX2, and ALP) with transfection of miR-34b-5p mimic or STAC2 siRNA under 7-day low-frequency SEMFs stimulation. e Quantitative analysis of osteogenic protein and STAC2 expression. Data represent mean ± SD; N = 3/group; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant by one-way ANOVA.

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