Fig. 11: Odor-delivery device and its equilibration.

A Schematics of the developed odor-delivery device. NO normally open (no stimulus) and NC normally closed (during stimulus). The insect was placed at 1 mm after EV9. B–E Testing of equilibration times and source stability. B PID responses to 0.5 s-linalool stimuli with different equilibration times. When the equilibration was too short, the PID response exhibited a transient peak. C With an equilibration of approximately 10 s the peak was no longer present, and the amplitude of the response did not change significantly with longer equilibration times. D Number of spikes recorded during 200 ms in response to 100 pg of Z7-12:Ac for different equilibration times. Each ORN was presented with 5 stimuli with different equilibration times (1, 3, 10, 30, and 79 s) either in increasing or decreasing order. For each order, the line is an average of 3 ORNs. The black line is the average of all 6 ORNs. E We measured the stability of the pheromone source first by applying 9 stimuli with 0.1 ng of Z7-12:Ac. Each stimulus was preceded by an equilibration time of 30 s. The inter stimulus interval was 2 min. Each line represents the response of a single ORN.