Fig. 1: Ovol2 deficiency results in poor heart formation and embryonic lethality.
From: Placental extracellular vesicles promote cardiomyocyte maturation and fetal heart development
a RT-PCR showing Ovol2 and Rn18s in the mouse embryo and placenta on E9.5. b Immunohistochemistry showing Troponin T (TnT; green, upper left images) and Cytokeratin (CK; green, upper right images) in the E9.5 embryo and placenta, respectively. DAPI was used to stain nuclei. In the bottom panels, in situ hybridization was used to demarcate Ovol2 (pink) expression in the embryo and placenta. Boxes in the lower magnification images (left panels) show the location of higher magnification images (right panels) for both the embryo and placenta. Please note that Ovol2-positive cells are only detectable within the placenta. c Phase-contrast images of Ovol2+/+ (WT), Ovol2+/- (Het) and Ovol2-/- (Null) embryos. Images were captured using a Leica DMi1 inverted microscope. Arrowheads indicate the fetal heart. d Immunohistochemistry showing TnT (green) in WT, Het, and Null embryos. Boxes in the lower magnification images (top panels) show the location of higher magnification images (bottom panels). e Quantification of the percent of WT, Het, and Null embryos present at various gestational timepoints (n = 4 pregnant mice; actual number of embryos/pups of each genotype are presented in Supplementary Table 1). Scale bars represent 500 µm for low magnification images in (b) and (d), and the images in (c). Scale bars for high magnification images in (b) and (d) represent 50 µm. Uncropped images of DNA gels are provided in Supplementary Fig. 7.
