Fig. 4: Placental EVs are present in fetal and maternal plasma.
From: Placental extracellular vesicles promote cardiomyocyte maturation and fetal heart development
a Schematic showing experimental design. Plasma was isolated from non-pregnant virgin mice, pregnant dams, or fetuses on E18.5. Plasma was then incubated with syncytin-A-FITC and CD9-PE antibodies, and subjected to nanoflow cytometry analysis. b Quantification of plasma EVs positive for both syncytin-A-FITC and CD9-PE antibodies. c Representative nanoflow cytometry plots showing an unstained control (pooled plasma), and syncytin-A-FITC and CD9-PE positive EVs isolated from plasma from non-pregnant virgin mice, pregnant dams, or fetuses on E18.5. Values significantly different from non-pregnant mice (N = 3 different mice, P < 0.05) are denoted with an asterisk (*), using one-way ANOVA followed by Tukey’s post-hoc test. Graphs represent means ± SEM.
