Fig. 4: Bone morphometric parameters in Clec14a−/− male mouse tibia. | Communications Biology

Fig. 4: Bone morphometric parameters in Clec14a−/− male mouse tibia.

From: Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning

Fig. 4

A) Diagram illustrating morphometry analyses timepoints (generated in BioRender): Tibiae were collected from C57BL/6J male mice at different skeletal maturation stages: juvenile (2- and 4-weeks-old), sexually mature (8-weeks-old) and mature adult (30-week-old). B Body weight. C Total tibiae length and D tibiae length from the proximal growth plate to the tibiofibular junction - PGP TFJ. Results for graphs B-C presented as mean ± SEM, data analysed with two-way ANOVA, followed by Dunnett’s post hoc. Results for graph D presented as median ± IQR, data analysed with multiple Mann–Whitney tests. E Methodology summary for the in vitro study of metatarsal elongation of E14.5 embryos (generated in BioRender). LAA-L ascorbic acid, βGP- β glycerophosphate. F Quantitation of metatarsal length as percentage increase. Results are presented as median ± IQR, data was analysed with Multiple Mann–Whitney tests. Data was obtained from N = 9, n = 34–40 Clec14a+/+ (WT) and N = 5, n = 13–16 Clec14a−/−, where N = individual biological replicates and n= total number of metatarsals analysed. G Representative μCT analysis images of 4- and 30-week-old Clec14a+/+ (WT) and Clec14a−/− mouse tibias. H Micro-computed tomography (μCT) analysis of trabecular percent bone volume (BV/TV %), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp). For BV/TV %, results are presented as median ± IQR, data was analysed with multiple Mann–Whitney tests. For the other parameters, results are presented as mean ± SEM, data was analysed with two-way ANOVA, followed by Dunnett’s post hoc, compared to WT mice. I Representative μCT images of trabecular bone extent (blue). Note the increased trabecular bone extent in Clec14a−/− samples. J Quantification of trabecular bone extent as a percentage. For all graphs, statistical test results are shown as: *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001. Sample size varies - each dot represents one biological repeat.

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