Fig. 4: Effect of SMJ-2 on respiratory metabolism.
A Increased resorufin fluorescence following treatment shows the accumulation of NADH/H+ in S. aureus ATCC 29213; values are in relation to the control, polymyxin B was used as positive control, and its MIC is 32 µg mL−1. B mRNA expression of ndh-2 gene in SMJ-2 treated and untreated sample of S. aureus 29213 strain, C Effects of SMJ-2, SMJ-4, and carbonyl cyanide m-chlorophenylhydrazine (CCCP) on the breakdown of iodonitrotetrazolium chloride (INT) into its detectable formazan product with a 490 nm absorbance. CCCP, a known electron uncoupler, was used as a positive control; the MIC of CCCP is 0.5 µg mL−1. The findings of each experiment are the average standard deviation of three individual repetitions (n = 3 biologically independent samples). Results were marked as significant (*) when P < 0.05 and highly significant (**) when P < 0.01, (***) when P < 0.001, and (****) when P value < 0.0001, and (ns) means non-significant. P value was calculated using a 95% class interval and paired t-test.
