Fig. 2: RABIF promotes HCC proliferation.
From: RABIF promotes hepatocellular carcinoma progression through regulation of mitophagy and glycolysis
A CRISPR/Cas9-mediated knockout of RABIF was confirmed by western blot in Huh7 and SK-Hep1 cells. B Cell proliferation was determined by CCK-8 assay. C Representative images (left) and quantification of colonies (right) from control and RABIF KO cells (n = 3 per cell type). D Overexpression of RABIF in Huh7 and SK-Hep1 cells was validated by immunoblot assay. E Cell proliferation of control and RABIF overexpression cells was compared by CCK-8 assay. F Representative images (left) and quantification of colonies (right) from control and RABIF overexpression cells (n = 3 per cell type). G–L Tumor growth curve (G, J), xenograft tumors (H, K) and tumor weight (I, L) in the subcutaneous implantation mouse model (n = 7 for each group). B, E, G, J Two-way ANOVA; (C, F, I, L) Unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001.
