Fig. 1: The workflow of 3D-Aligner.
From: 3D-Aligner: advanced computational tool for correcting image distortion in expansion microscopy
a Representative 12-fold ExM images of an RPE1 nucleus labeled for DNA and kinetochores experiencing sample drift. White arrows denote the direction of the drift and blue dotted line represents the nuclear boundary. b Images of xy, xz, and yz views of the drifted RPE1 nuclei in a showing the impact of sample drift on 3D image reconstruction. c Diagram showing the 3D-Aligner workflow. (1) Import images, (2) choose options for feature detection, (3) perform feature detection, (4) match features and calculate drift, (5) perform image drift correction, and (6) export corrected image data. d Snapshots of the 3D-Aligner interface and workflow. e Representative images showing feature detection, feature matching, drift calculation, and image drift correction between adjacent z planes. Stars mark detected features from background signals.
