Fig. 2: Effects of growth stages and phage on expression of wza and luxR regulating phage adsorption.

A, B The relative expression of AJ01 luxR and wza at different growth stages (a, b, c and d) and growth stage a infected with phage (EES-P), 16 s rRNA was the internal reference gene. C Adsorption rate of vB_VspM_VS2 by its host strain V. splendidus of different growth stages (a, b, c and d) and EES-P. D: Tenfold serial dilutions of 2 μL not adsorbed vB_VspM_VS2 plated on wild-type V. splendidus. E–I SEM images V. splendidus cells of different growth stages (a, b, c and d) and EES-P for 12 h. J Growth curves of AJ01 strain in 2216E medium. Optical densities (OD₆₀₀) of cultures of AJ01 wild-type (WT) in the presence phage vB_VspM_VS2 at a multiplicity of infection (MOI) of 1. K Corresponding abundances of pfu/mL were quantified by plaque assay over a 16-h period of incubation in AJ01 cultures in the presence of phage vB_VspM_VS2 at a multiplicity of infection (MOI) of 0.1 and 1 were measured at different incubation times, respectively. Error bars represent standard deviations from all experiments carried out in duplicate. P < 0.05; **, P < 0.01; ***,P < 0.001; #, P > 0.05. L The abscissa in the figure is the ratio of the number of differential genes annotated on GO Term to the total number of differential genes. The ordinate is GO Term. The size of the dot represents the number of genes annotated on GO Term. The color from red to purple represents the significance of enrichment. The figure represented the comparison V. splendidus (V.s) between high-cell-density state and low-cell-density state. M The abscissa in the figure is the KEGG pathway, and the ordinate is the significance level of pathway enrichment. The higher the value is, the more significant it is. The figure represented the comparison V. splendidus (V.s) between high-cell-density state and low-cell-density state.