Fig. 1: Igf2bp1 depletion resulted in spermatogenic defects in an age-dependent manner in mice.
From: RNA-binding protein IGF2BP1 is required for spermatogenesis in an age-dependent manner
a Schematic strategy for constructing the Igf2bp1 conditional knock-out (cKO) mice. b Western blotting analysis of IGF2BP1 expression in the testes of wildtype (WT) and Igf2bp1 cKO mice. GAPDH was used as a loading control. c The morphology of testes from 8-month-old WT and Igf2bp1 cKO mice. Scale bar: 1 cm. d The testis-to-body weight ratios of 2-, 4-, 8- and 12-month-old WT and Igf2bp1 cKO mice (n = 3). Bar graphs represent the means ± standard deviations. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001. e, f Representative images of periodic acid-Schiff-stained testicular sections from 2-, 4-, 8- and 12-month-old WT and Igf2bp1 cKO mice (n = 3). Scale bar: 100 µm. The black asterisks indicate abnormal seminiferous tubules. Bar graphs represent the means ± standard deviations. ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. g Representative images of hematoxylin-eosin stained cauda epididymal sections from 2-, 4-, 8- and 12-month-old WT and Igf2bp1 cKO mice. Scale bar: 100 µm. h The sperm concentration per cauda epididymis from 2-, 4-, 8- and 12-month-old WT and Igf2bp1 cKO mice (n = 3). Bar graphs represent the means ± standard deviations. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
