Fig. 1: AR expression and activity are elevated in bPSC freshly isolated from inflamed prostates and the enhanced AR activity is sustained in inflamed organoids in the absence of androgen. | Communications Biology

Fig. 1: AR expression and activity are elevated in bPSC freshly isolated from inflamed prostates and the enhanced AR activity is sustained in inflamed organoids in the absence of androgen.

From: Inflammation impacts androgen receptor signaling in basal prostate stem cells through interleukin 1 receptor antagonist

Fig. 1: AR expression and activity are elevated in bPSC freshly isolated from inflamed prostates and the enhanced AR activity is sustained in inflamed organoids in the absence of androgen.

A Representative flow cytometry dot plots and histograms of intracellular AR in bPSC from naïve and inflamed prostates. B Bar graph showing the percentage of AR+ cells within naïve and inflamed bPSC population as analyzed with flow cytometry (Naïve, n = 10; Inflamed, n = 5). C Immunoblot of full-length AR in naïve and inflamed bPSC lysates. D Fold change of AR target genes significantly upregulated in inflamed bPSC (Naïve or Inflamed, n = 3). E qRT-PCR of conventional AR target genes, Tmprss2 and Nkx3-1, in bPSC. Matched luminal population is included as a control (Naïve or Inflamed, n = 3). F Immunoblot showing increased level of full-length AR in inflamed organoids compared to naïve organoids. Full-length AR levels in both naïve and inflamed organoids were elevated with 1 nM R1881 treatment. G AR immunofluorescence staining in organoids derived from naïve or inflamed bPSC. Representative cells with nuclear-localized AR are indicated with white arrows. AR nuclear localization is also shown in the inset image with higher magnification (Scale bar, 100 μm). H Fold change of AR target genes significantly upregulated in inflamed organoids (Naïve or Inflamed, n = 3). Data presented in this figure are mean ± SEM.

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