Fig. 1: Schematic representation of the study. | Communications Biology

Fig. 1: Schematic representation of the study.

From: Stage-specific expression patterns and co-targeting relationships among miRNAs in the developing mouse cerebral cortex

Fig. 1

To investigate the expression patterns of miRNAs during cerebral cortical development in the mouse, we performed RNA-seq of bulk tissue at E14, E17 and P0 as well as in NPCs isolated from the cerebral cortex and differentiated into neurons. Subsequently, we employed weighted gene co-expression network analysis (WGCNA) to identify modules of co-expressed miRNAs. We then constructed a network of miRNAs sharing more target genes than expected by chance. Co-targeting relationships between selected miRNAs were validated using luciferase reporter assays, demonstrating enhanced gene silencing effect on neurodevelopmentally-relevant genes for combinations of miRNAs compared to individual miRNAs.

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