Fig. 10: Pulldown of the native PfKelch13 protein from P. falciparum total protein extracts using hemin-agarose beads. | Communications Biology

Fig. 10: Pulldown of the native PfKelch13 protein from P. falciparum total protein extracts using hemin-agarose beads.

From: Artemisinin-resistant Plasmodium falciparum Kelch13 mutant proteins display reduced heme-binding affinity and decreased artemisinin activation

Fig. 10

3D7 parasites were isolated by saponin treatment from asynchronized culture and proteins were solubilized with RIPA buffer. Protein extracts were incubated with hemin-agarose or glutathione (GSH)-agarose beads. After extensive washing, bound proteins were eluted with Laemmli buffer, separated by SDS-PAGE, and blotted against PfKelch13 antibodies. PfKelch13 antibodies were custom-generated using a PfKelch13 peptide51. Molecular weight standards (in kDa) are as indicated.

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