Fig. 4: Quantitative evaluation of TrK13- heme binding by MST. | Communications Biology

Fig. 4: Quantitative evaluation of TrK13- heme binding by MST.

From: Artemisinin-resistant Plasmodium falciparum Kelch13 mutant proteins display reduced heme-binding affinity and decreased artemisinin activation

Fig. 4

A–D Dose-response curves showing negative amplitude of response for TrK13-WT (A), TrK13-R539T (B), TrK13-C580Y (C) and TrK13-A578S (D) titrated against increasing concentrations of heme. MST experiments were performed at medium MST power at 25 °C. LED power was set to 60% (A and D) or 40% (B and C) excitation. E Comparative dose-response curves for the binding interaction between the four TrK13 variants and heme, as indicated. The protein concentration was kept constant at either 50 nM (TrK13-WT or TrK13-A578S) or 25 nM (TrK13-R539T or TrK13-C580Y), while the ligand heme concentration varied from 0-5 µM. F Table showing the decreasing affinity scale: TrK13-WT > TrK13-A578S > TrK13-R539T > TrK13-C580Y and signal-to-noise (S/N) ratio.

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