Fig. 6: LINC02139 is positively correlated with XIAP in the human GC samples and participates in mediating GC cell apoptosis. | Communications Biology

Fig. 6: LINC02139 is positively correlated with XIAP in the human GC samples and participates in mediating GC cell apoptosis.

From: LINC02139 interacts with and stabilizes XIAP to regulate cell proliferation and apoptosis in gastric cancer

Fig. 6: LINC02139 is positively correlated with XIAP in the human GC samples and participates in mediating GC cell apoptosis.The alternative text for this image may have been generated using AI.

A XIAP protein expression in 12 pairs of human GC samples was detected using western blotting, normalized to GAPDH. The relative protein expression level was quantified by comparing the gray level of each band. N, normal; T, tumor. *P > 0.05; ****P < 0.001. B LINC02139 expression in the 12 pairs of human GC samples was measured using qRT-PCR. Normalized to GAPDH. N, normal; T, tumor. *P > 0.05; **P < 0.05, ***P < 0.01, ****P < 0.001. C A Pearson correlation analysis was performed to determine the correlations between LINC02139 and XIAP expression. D A fluorescent microscopy technique was applied to visualize the Hoechst 33258 staining in the nucleus of the indicated transfected GC cells. Arrows in the images indicate the cells with condensed chromatin and fragmented nuclei. The apoptotic ratios were displayed. ***P < 0.01, ****P < 0.001. E Flow cytometry assays were used to inspect the apoptosis in the transfected GC cells. The apoptotic ratios were shown as histograms. ****P < 0.001. Scale bars, 50 μm in (D).

Back to article page