Fig. 1: Cryo-EM structure of RV-B14 virions at 2.9 Å resolution. | Communications Biology

Fig. 1: Cryo-EM structure of RV-B14 virions at 2.9 Å resolution.

From: Cryo-EM of human rhinovirus reveals capsid-RNA duplex interactions that provide insights into virus assembly and genome uncoating

Fig. 1

A Cryo-EM image of RV-B14. Arrows indicate empty capsids. Bar = 250 Å. B Radially color-coded surface representation of RV-B14 outer (left) and inner (right) surfaces viewed along a two-fold axis. Ordered, genomic RNA at the inner surface of the RV-B14 capsid beneath the two-fold axes (pink). The color key (center) indicates the radial distance (in Å) from the particle center. In these images the capsid density is sharpened, whereas dsRNA density is unsharpened. Symbols indicate icosahedral symmetry axes. C A 5S protomer (or asymmetric unit) viewed from the inside of the capsid, showing the ordered regions of VP1 in blue, VP2 in green, VP3 in red, and VP4 in yellow. Ordered N and C termini are indicated. The N-terminal segment of VP1 Glu7-Lys30 is highlighted in light blue. D Sharpened cryo-EM density of a dsRNA helical segment (left), fitted with an atomic model of a 13bp-dsRNA segment and two pendant bases (blue slabs for bases) (center & right images). Arrows indicate the two terminal unpaired nucleotides at each end of the dsRNA.

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