Fig. 4: Losartan treatment blocks vessel migration in HF but not sham rats.

a Schematic illustration of losartan administration via drinking water in sham and HF rats. For two weeks after the surgery, losartan (20 mg/kg) was supplied in the drinking water. Following echocardiographic assessment, rats were treated for two more weeks before tissue collection and immunohistochemical staining and analysis. b Quantification of the number of vessel-associated and parenchymal microglia after losartan treatment in sham rats (n = 8 per group). c Confocal images show vessel-associated microglia with and without losartan treatment in HF rats at two different Bregma levels, scale bar 100 µm. d Quantification of the number of vessel-associated and parenchymal microglia after losartan treatment in HF rats (n = 8 rats per group). e Pie charts show the relative percentages of Type I–III vessel-associated microglia with and without losartan treatment in HF rats.