Fig. 4: G3BP1 is largely occupied by RNA with m6A modification by σB of NDRV HN10. | Communications Biology

Fig. 4: G3BP1 is largely occupied by RNA with m6A modification by σB of NDRV HN10.

From: Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings

Fig. 4: G3BP1 is largely occupied by RNA with m6A modification by σB of NDRV HN10.

a Overview of four populations of genes with differential G3BP1 occupancy and m6A modification (log2 FC > 1 or <−1, P < 0.05) represented by different colors. The “73.5%“ is highlighted for the transcripts with high G3BP1 enrichment and m6A modification by NDRV HN10 compared to NC. b Heatmap showing the top 10 activity scores of GO analysis in these four populations ranked by t values. K-S test-like rank statistics of the gene set in each pathway were calculated, and the expression matrix was converted into a pathway enrichment score matrix to obtain the activity score. The difference in each population’s activity score compared to all other populations was analyzed using the “limma” package, and t values of the top 10 terms with P values less than 0.05 were used to construct the heatmap. The four colors indicate the same population as in (a). c m6A distribution of the RNAs indicated by the same four colors in (a). RNAs with upregulated enrichment of G3BP1 and m6A in NDRV HN10 compared with NC had high m6A modification at the 5′ UTR. d Venn diagram showing the intersection between G3BP1-enriched RNAs in vivo and Flag (σB)-enriched RNAs in vitro. e m6A distribution of σB- and λA-enriched RNAs. RNAs with σB occupancy have high m6A modification at the 5′ UTR. NC negative control, FC fold change, UTR untranslated region, CDS coding sequence.

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