Fig. 5: Transcription of sulfur oxidation genes in H. denitrificans strains lacking soxT1B.

RT-qPCR analysis is shown for four indicative genes in three different H. denitrificans ΔsoxT1B strains in the absence (a) and in the presence of 2 mM thiosulfate (b). The ability of the strains to oxidize thiosulfate is indicated. The growth experiments are shown in full in Supplementary Fig. 7. All strains grew equally well on methanol in the absence of thiosulfate (Supplementary Fig. 6a). Three parallel experiments were performed to obtain the averages and standard deviation. Data are presented as means ± SD. Individual data points are indicated. One-way ANOVA was performed to calculate p-values. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, ns = (p > 0.05)). Color coding: black: differences compared to strain ΔtsdA in the absence of thiosulfate; blue, differences compared to ΔtsdA in the presence of thiosulfate; red, differences compared to the same strain in the absence of thiosulfate. TS thiosulfate, ns not significant.