Fig. 1: Generation of midbrain-striatum assembloid model with identity specificity.
From: Modeling early phenotypes of Parkinson’s disease by age-induced midbrain-striatum assembloids
a Schematic representation of the assembloid model generation. b Representative confocal image of a 70 μm assembloid section immunostained with Hoechst, FOXA2 visible in the MO side and CTIP2 visible in the StrO side of the assembloid. GFP fluorescence is intrinsic in the midbrain part of the assembloids. c Representative confocal image of a 70 μm assembloid section immunostained with Hoechst and ASCL1 visible in the StrO side of the assembloid. GFP fluorescence is intrinsic in the MO part of the assembloids. d Representative confocal image of a 70 μm assembloid section immunostained with Hoechst and CORIN visible in the MO side of the assembloid. GFP fluorescence is intrinsic in the midbrain part of the assembloids. e Representative confocal image of a 70 μm assembloid section immunostained with Hoechst and TH visible in the MO side of the assembloid. GFP fluorescence is intrinsic in the midbrain part of the assembloids. f Representative confocal image of a 70 μm assembloid section immunostained with Hoechst and DARPP32 visible in the StrO side of the assembloid. GFP fluorescence is intrinsic in the midbrain part of the assembloids. g Representative brightfield image of an assembloid in a well of the 48-well MEA plate. Plots showing the quantification of the Number of Spikes, Inter-spike Interval in seconds and the Burst Frequency in Hz between assembloids generated from two independent human WT cell lines. The data in the plots represent recordings of individual assembloids for the culture periods D32 to D43, from 3 to 4 batches. Batch correction was applied by normalising each value to the mean of the values for each batch. Outliers were calculated in GraphPad Prism using the ROUT method Q 1%. Two-sided Wilcoxon test was performed in R 4.2.2.
