Fig. 6: Dynamic binding of miR-276 and miR-182013-5p to Jhamt. | Communications Biology

Fig. 6: Dynamic binding of miR-276 and miR-182013-5p to Jhamt.

From: miR-276 and miR-182013-5p modulate insect metamorphosis and reproduction via dually regulating juvenile hormone acid methyltransferase

Fig. 6

Temporal expression patterns of miR-276 (a) and miR-182013-5p (b) in the corpus allatum. N4, 4th instar nymphs; N5, 5th instar nymphs; E, early (day 1); M, middle (day 2 for N4, and day 3 for N5); L, late (day 4 for N4, and day 5 for N5). Different letters indicate significant differences at P < 0.05. n = 6. c Diagram illustrating ChIRP procedure for measuring miRNA binding to Jhamt. Enrichment of miR-276 and miR-182013-5p from Jhamt in the 4th instar nymphal (d) and adult (e) stages. *P < 0.05; **P < 0.01; ***P < 0.001. n = 3–4.

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