Fig. 1: Explanation of the assay.
From: Coordination of transporter, cargo, and membrane properties during non-vesicular lipid transport
A Schema of the two overlapping laser foci of the confocal microscope, which form a light cuvette. Transition of the fluorescent LUVs and of the lipid biosensors are visualized as signal fluctuations. B Example of fluctuating green and red signals with a high level of cross-correlation Gcc(0). C FCCS read-out, Gcc(0)/GR(0), as a function of cargo lipid in labeled LUVs (black squares). The two regimes of the transport experiments: (i) extraction (red solid line), and (ii) release (red dotted line). The blue dotted line stands for the initial change in Gcc(0)/GR(0) due to extraction immediately upon Osh6 addition before the release regime starts, which occurs upon the Osh6 addition.
