Fig. 7: Synchronization of charged lipids and cargo occupancy required for the PI4P extraction from the PM-like membrane.
From: Coordination of transporter, cargo, and membrane properties during non-vesicular lipid transport
A Comparison of PI4P extraction by Osh6 from LUVs A that are otherwise neutral (left), and rich in PS (right). Osh6 was preincubated with LUVs B, which (i) supply Osh6 with PS, and (ii) in the final mixture, serve space for PI4P release. LUVs B were composed of POPC/PS (20 mol%, blue curves), POPC (red curves) or were absent (black curves). B Extracted fraction of PI4P within first 10 min of the experiment for compositions of LUVs A and LUVs B given in (A). C Extraction rate of PI4P from the LUVs containing other charged lipids. D Extraction rate of PS from the LUVs containing other charged lipids. Composition of the experiment in 6 A, B: cPI4P = 500 nM, cSidC-Atto488 = 100 nM, ctotal lipids LUV A = 50 µM, ctotal lipids LUV B = 200 µM; in 6 C: cPI4P = 500 nM, cOsh6 = 250 nM, cSidC-Atto488 = 100 nM, ctotal lipids LUV A = 50 µM; in 6D: cPS = 500 nM, cOsh6 = 250 nM, cC2lact-CFP = 50 nM, ctotal lipids LUV A = 50 µM. All error bars represent the standard error of the mean, n = 10 measurements. The p-values were obtained from the two-sample t-test.
