Fig. 2: Transcriptomic analysis of male and female larvae exposed to B vitamins.

A Experimental set-up: mosquito larvae reared in gnotobiotic conditions with the auxotrophic E. coli strain were decolonized at the beginning of the third instar (L3) to obtain germ-free larvae. Larvae were either kept in germ-free conditions or treated with 8× B vitamin solutions. After three to six hours since moulting to the fourth instar (L4), larvae were individually collected. DNA was extracted from individual larvae for sex assignment using the Nix gene, while RNA was extracted for transcriptome sequencing on male and female larvae. A more precise version of the experimental set up can be found in Supplementary Fig. 2. B–E Reads Per Kilobase per Million mapped read (RPKM) values of representative genes differentially regulated with B vitamins in males (B, C) and females (D, E). Bar charts represent the mean ± SEM. Genes are named according to their Vectorbase ID.