Fig. 5: EZH2 promotes VEGFC secretion in ESCC and thereby inhibits DC maturation. | Communications Biology

Fig. 5: EZH2 promotes VEGFC secretion in ESCC and thereby inhibits DC maturation.

From: EZH2 elicits CD8+ T-cell desert in esophageal squamous cell carcinoma via suppressing CXCL9 and dendritic cells

Fig. 5

A RNA-seq was performed after EZH2 knockdown in KYSE150 cells and the down-regulated genes associated with secretory cytokines were shown according to the requirement: |Log2(Foldchange)|>0.5, P < 0.05. B The levels of VEGFC secreted by either KYSE150 or KYSE510 cells after EZH2 knockdown were detected by ELISA. C, D Flow cytometry was used to determine monocytes differentiated into DCs by anti-CD14, anti-CD80 and anti-CD86 staining for PBMC and DCs. E The percentage of induced mature DC cells was measured by using flow cytometry while culture supernatants from either KYSE150 or KYSE510 cells with EZH2 knockdown, or the supernatants supplemented with VEGFC, were incubated with the differentiated DCs. The above results represent the mean ± standard deviation (M ± SD). n = 3 independent biological replicates.

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