Fig. 3: T. gondii infection induces MYR1-dependent DNA damage foci early after infection. | Communications Biology

Fig. 3: T. gondii infection induces MYR1-dependent DNA damage foci early after infection.

From: Toxoplasma gondii infection induces early host cell cycle arrest and DNA damage in primary human host cells by a MYR1-dependent mechanism

Fig. 3

A, B HUVEC (n = 3) were infected with tachyzoites (MOI 0.5:1) of TgΔhce1, TgΔmyr1 mutants and control parasites (TgΔku80), fixed at 15 min, 1 and 3 h p. i. and stained for γH2AX (cyan) to quantify DNA damage foci. Tachyzoites were detected by specific antibodies (red) and the nuclei were stained by DAPI (blue). B The percentage of DNA damage-positive cells was quantified in comparison to the total number of cells in the field of view at 15 min, 1 h and 3 h p. i. Data show that T. gondii infection-driven double-strand DNA breaks occur already at 15 min p. i. in a MYR1-dependent manner. The p-values were calculated using a one-way ANOVA followed by a Kruskal Wallis multiple comparison test of variance, using the non-infected condition (n. i.) as control. A p < 0.05 was considered as significant.

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