Fig. 4: T. gondii-driven DNA damage in relation to host cell cycle phases. | Communications Biology

Fig. 4: T. gondii-driven DNA damage in relation to host cell cycle phases.

From: Toxoplasma gondii infection induces early host cell cycle arrest and DNA damage in primary human host cells by a MYR1-dependent mechanism

Fig. 4

A, B HUVEC (n = 3) were infected with tachyzoites (MOI 0.5:1) of TgΔhce1, TgΔmyr1 mutants and control parasites (TgΔku80). B T. gondii-infected cells (3 h p. i.) were stained in parallel for γH2AX (DNA damage foci marker), geminin and PCNA to study whether DNA damage foci formation was linked to a distinct cell cycle phase. γH2AX-positive cells were assigned to each cell cycle phase and plotted as the percentage of DNA damage-positive cells. The scale bar represents 5 µm. Graph bars represent the median ± SD of three biological donors. The p-values were calculated using a one-way ANOVA followed by a Kruskal Wallis multiple comparison test of variance, using the non-infected condition (n. i.) as control. A p < 0.05 was considered as significant.

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