Table 1 Antibodies used in the current study
Antigen | Company | Cat. number | Origin/reactivity | Dilution |
|---|---|---|---|---|
Primary antibodies IFA | ||||
Histone H3 (S10) | Abcam | Ab5176 | Rabbit | 1:300 |
H2AvD (pS139) | Cell Signalling | 9718S | Rabbit | 1:200 |
Histone H2A.X (pS139) | Cell Signalling | 80312S | Mouse | 1:400 |
PCNA | Abcam | ab18197 | Rabbit | 1:200 |
Geminin | Cell Signalling | 52508 | Rabbit | 1:800 |
Toxoplasma gondii | Thermo Fisher | PA1-7256 | Goat | 1:100 |
β-catenin | Abcam | ab32572 | Rabbit | 1:200 |
Primary antibodies WB | ||||
Vinculin | Santa Cruz | sc-73614 | Mouse | 1:1000 |
HR DNA repair antibody sampler kit | Cell Signalling | 99891 | Rabbit | 1:1000 |
MNR complex Antibody Sampler kit | Cell Signalling | 8344 | Rabbit | 1:1000 |
Secondary antibodies | ||||
|---|---|---|---|---|
Antigen/conjugate | Company | Cat. number | Host/target | Dilution |
Goat anti-mouse IgG Peroxidase conjugated | ||||
Goat anti-rabbit IgG Peroxidase conjugated | Pierce | 31430 | goat/mouse | 1:40,000 |
Goat anti-mouse IgG Peroxidase conjugated | Pierce | 31460 | goat/rabbit | 1:40,000 |
Alexa Fluor 488 | Thermo Fisher | A11001 | goat/mouse | 1:500 |
Alexa Fluor 488 | Thermo Fisher | A11008 | goat/rabbit | 1:500 |
Alexa Fluor 594 | Thermo Fisher | A21468 | chicken/goat | 1:500 |
Alexa Fluor 647 | Thermo Fisher | A21244 | Goat/rabbit | 1:500 |
Alexa Fluor 647 | Thermo Fisher | A21235 | Goat/mouse | 1:500 |
