Fig. 2: Spatial collection of organelle material and analysis of mitochondrial respiration from Aβ-deposit-exposed SIM-A9 cells.

a Cells were exposed to pre-aggregated peptides or solvent control (PBS). Sample images were taken after 24 h. Scale bar: 25 µm. Peptide deposits have been artificially stained black for better visualization (original images provided in Suppl. Figure 1). b After 24 h exposure, mitochondrial and nucleic material was collected from cells in close proximity (close, C) to peptide deposits or further away (far, F) after staining of mitochondria as indicated in the scheme. Far and near distances were defined using the half-maximum distance between two neighboring peptide deposits, selecting the 10% distance near the deposit and the 90-100% of the radius away from the deposit. Distances to the edge of the deposits are expressed as mean + SD (n = 36 per group, values derived from all samples aspirated). Brown–Forsythe ANOVA test with Dunnett’s T3 multiple comparisons test was performed for statistical analysis (***p < 0.001). The box plot indicates min and max. c Oxygen consumption rate was measured in freshly sampled mitochondrial material (ScAβ: scrambled peptide; Aβ: Aβ peptide). Four independent experiments were performed (n = 4). Each sample consisted of material from three cells ejected in 6 µL buffer. Random samples were taken for the PBS control. Statistical analysis was performed by one-way ANOVA followed by Sidak’s multiple comparison post-test (*p < 0.05; ns, p > 0.05).