Table 1 Cryo-EM data collection, refinement, and validation statistics
Delta S protein/REGN10987-Fab (EMDB-14750) (PDB 7ZJL) | |
|---|---|
Data collection and processing | |
Magnification | 130,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 50 |
Defocus range (μm) | −0.8 to −1.6 |
Pixel size (Å) | 0.929 |
Symmetry imposed | C1 |
Initial particle images (no.) | 147,977 |
Final particle images (no.) | 44,323 |
Map resolution (Å) | 2.53 |
FSC threshold | 0.143 |
Map resolution range (Å) | 2.0–9.0 |
Refinement | |
Initial model used (PDB code) | 7SBL, 6XDG |
Model resolution (Å) | 2.60 |
FSC threshold | 0.143 |
Model resolution range (Å) | 2.0–9.0 |
Map sharpening B factor (Å2) | −36.6 |
Model composition | |
Non-hydrogen atoms | 35,140 |
Protein residues | 4551 |
Ligands | 0 |
B factors (Å2) | |
Protein | 74.33 |
Ligand | |
R.m.s. deviations | |
Bond lengths (Å) | 0.012 |
Bond angles (°) | 1.847 |
Validation | |
MolProbity score | 0.81 |
Clashscore | 0.07 |
Poor rotamers (%) | 0.48 |
Ramachandran plot | |
Favored (%) | 95.95 |
Allowed (%) | 4.01 |
Disallowed (%) | 0.04 |
