Fig. 2: Energy expenditure decreases after Methylene blue exposure in zebrafish larvae.

Schematic representation detailing the experimental design for A 0–1 dpf, C 0–4 dpf, and E transient 3–4 dpf MB exposure. B Energy expenditure was measured using the Alamar Blue assay in 1 dpf embryos at 3, 6, 12, and 24 h. D Energy expenditure was measured using the Alamar Blue assay in 4 dpf larvae at 24 and 48 h. F Energy expenditure was measured using the Alamar Blue assay in 4 dpf larvae at 24 after transient 3–4 dpf MB exposure. The data were graphed as violin plots showing the mean (symbol: +), the median represented by a line in the middle, the upper and lower quartile range indicated by the dotted lines, and the outline of the violin plot illustrates the kernel probability density. n = 10–12 (Each biological replicate is represented by a single embryo), Asterisk indicates *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.