Fig. 2: Impact of bead truncation on UMI deduplication accuracy. | Communications Biology

Fig. 2: Impact of bead truncation on UMI deduplication accuracy.

From: Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences

Fig. 2: Impact of bead truncation on UMI deduplication accuracy.The alternative text for this image may have been generated using AI.

a Generation of a sequencing library from 10x bead oligos and then sequenced using ONT flongle. b A library was generated from oligos on Drop-seq beads and then sequenced using ONT flongle. c Visualisation of the top 20 overrepresented UMIs in the 10x Chromium 5k PBMC v3.1 dataset, displaying the count of each UMI. d Visualisation of the top 20 overrepresented UMIs in the Macosko et al.1 dataset, displaying the number of UMI counts.

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