Fig. 5: Including an anchor into the beads increases the number of UMIs and transcripts detected.
From: Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences
a The log10 density of transcripts per cell for both positional and anchor UMI selection approaches. b The bar plot compares the percentage of cells expressing low ( < 500 transcripts), mid (500-1000 transcripts), and high ( > 1000 transcripts) numbers of features between the positional and anchor UMI selection approaches. The confidence categories for transcript counts are indicated by colour coding: low (white), mid (grey), and high (blue). Data is representative of one of three independent experiments. c Correlation of transcripts in human-origin cells between the positional and anchor approaches. Transcripts with enhanced detection using the anchor method are highlighted with a red circle. d Correlation of mouse-origin transcripts between the positional and anchor approach, with the red circle identifying transcripts more readily detected using the anchor UMI identification approach. e Expression profile of the transcript ENSMUST00000034270 for the positional and anchor approach. f UMAP plots showing the increased expression of ENSMUST00000034270 using the anchor approach. Each dot represents a single cell. The data shown is from one of three independent experiments. For plots (c–f), each dot represents a single cell. The data shown is from one of three independent experiments.
