Fig. 8: hnRNPA2B1 binds to the target gene ITGA2.
a mRNA binding to hnRNPA2B1 was predicted using the starBase database and transcriptomic sequencing. b The binding ability of ITGA2 to hnRNPA2B1 was predicted using the RPISeq database and RIP assay results. c The m6A site of ITGA2 was predicted using the SRAMP database. d MazF assay was used to analyse the m6A modification level of ITGA2 after mineralization. e Western blot and qRT‒PCR analysis of ITGA2 expression after 7 and 14 days of odontogenic differentiation. f qRT-PCR was used to determine the ITGA2 expression levels. g, h Expression of odontogenic differentiation-related mRNAs and proteins 7 days and 14 days after ITGA2 knockdown. i ALP and ARS staining results. j Western blot analysis revealed that the sh-ITGA2 group had lower levels of p-AKT and p-PI3K expression. k SC79 increased the expression of p-AKT and p-PI3K, according to Western blot analysis. l The impact of SC79 on the odontogenic differentiation of hDPSCs was detected using Western blot. b, d–l All data were presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: not significant, b, e–l n = 3 for each group, d n = 9, n = 6 for each group. i Scale bar, 200 μm.
