Fig. 4: Phenotypic analysis of mice with GATA4-BS1sub hemizygous of the mXYSRa/Enh13 region.
From: GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse
A A result of PCR direct sequence analysis of hemizygous GATA4-BS1sub. Electropherogram is shown. Substituted base is labeled with blue. B A result of PCR sexing of XY GATA4-BS1sub/mXYSRdel. An image of agarose gel electrophoresis is shown. Genes (X-linked Xist and Y-linked Zfy) detected are pointed at left. Genotypes are indicated at top. N: no template control; M: 100 bp DNA ladder marker. The full gel image is shown in Supplementary Fig. S7. C Phenotypes of GATA4-BS1sub hemizygous F0 individuals. Genotypes and sex chromosome compositions are indicated at top. T: testis; O: ovary.
