Table 1 Primers used in this study
From: GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse
Primer name | Sequence |
|---|---|
mXYSRa/Enh13_gRNA A_Fw | AGACTTGAAATTATTAAGAGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA A_Rv | AACTCTTAATAATTTCAAGTCTCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA A_Fw | TTAATACGACTCACTATAGGAGACTTGAAATTATTAAGA |
mXYSRa/Enh13_gRNA B_Fw | TCCTGTTTTGAAGCCCACCGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA B_Rv | AACGGTGGGCTTCAAAACAGGACGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA B_Fw | TTAATACGACTCACTATAGGTCCTGTTTTGAAGCCCACC |
mXYSRa/Enh13_gRNA C_Fw | AGTGGAAAGGTTTTCTCAGGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA C_Rv | AACCTGAGAAAACCTTTCCACTCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA C_Fw | TTAATACGACTCACTATAGGAGTGGAAAGGTTTTCTCAG |
mXYSRa/Enh13_gRNA D_Fw | CAGAGCACAGGGTTTGAAGGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA D_Rv | AACCTTCAAACCCTGTGCTCTGCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA D_Fw | TTAATACGACTCACTATAGGCAGAGCACAGGGTTTGAAG |
mXYSRa/Enh13_gRNA E_Fw | TTGTCTCAGCAGAGGCAATGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA E_Rv | AACATTGCCTCTGCTGAGACAACGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA E_Fw | TTAATACGACTCACTATAGGTTGTCTCAGCAGAGGCAAT |
mXYSRa/Enh13_gRNA F | CTAATACGACTCACTATAGTGAGACAATCATATCTTCCAGTTTTAGAGCTAGAAATAGCA |
mXYSRa/Enh13_gRNA G_Fw | CTCCTGGAATCCTTCTCCTGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA G_Rv | AACAGGAGAAGGATTCCAGGAGCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA G_Fw | TTAATACGACTCACTATAGGCTCCTGGAATCCTTCTCCT |
mXYSRa/Enh13_gRNA H_Fw | GCTGTGGGAGAGTCGCAGAGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA H_Rv | AACTCTGCGACTCTCCCACAGCCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA H_Fw | TTAATACGACTCACTATAGGGCTGTGGGAGAGTCGCAGA |
mXYSRa/Enh13_gRNA I_Fw | GTGTGTTCTAATCAGCCAGGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA I_Rv | AACCTGGCTGATTAGAACACACCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA I_Fw | TTAATACGACTCACTATAGGGTGTGTTCTAATCAGCCAG |
mXYSRa/Enh13_gRNA J_Fw | ATAACAAACATTTACTGATGTTTTAGAGCTAGAAATAGCAAG |
mXYSRa/Enh13_gRNA J_Rv | AACATCAGTAAATGTTTGTTATCGGTGTTTCGTCCTTTCCAC |
T7_mXYSRa/Enh13_gRNA J_Fw | TTAATACGACTCACTATAGGATAACAAACATTTACTGAT |
gRNA Rv | AAAAGCACCGACTCGGTGCC |
GATA4_BS_sub_SSODN1 | GCTCTGAAATCGTGCCCATTGCCTCTGCTGAGACAATCATrcCTTCCAAGGAGAAGGATTCCAGGAGCTGTGGGAGAGT |
GATA4_BS_sub_SSODN2 | ACTCTCCCACAGCTCCTGGAATCCTTCTCCTTGGAAGgyATGATTGTCTCAGCAGAGGCAATGGGCACGATTTCAGAGC |
XYSR 1F3 | CCCATGTACAGTTCACGCTTC |
XYSR 1 R | CAGCCCCATAATAAGCAAGG |
XYSR 6 F | CCTTTGCTACCCAAACCTCA |
XYSR 6 R | TTTGTGCGCAGACTATCAGG |
mXYSRa Fw1 | AATCACAAAAGGCACTGAGG |
mXYSRa Fw2 | TACATCTGCCCCAAGGAAGC |
mXYSRa Fw3 | AAGAAGGTAGACCCAGAGAC |
mXYSRa Rv6 | TGCTAGAATAAAGCTAAGCC |
mXYSRaTg Fw1 for cloning | ATAGCTGAGGAATTAGAAGG |
mXYSRaTg Rv1 for cloning | TGGCTTCCAATTCTCTGCGG |
Gata4F | GCGGTACCGTGACAGTTCCGCACACCCG |
Gata4R2 | GCGGATCCGGCTTTTCCAGTGCTCCACC |
SfiI_SalI_mXYSRa Fw2 | GCGGCCAAACAGGCCGTCGACAGAAGGTAGACCCAGAGACA |
SfiI_mXYSRa Rv2 | GCGGCCTGTTTGGCCTAAGCCAAATCTTGAAACAT |
mXYSRa Sal inverse PCR Fw1 | GCTTAGTCGACGGCCAAACAGGCCGCATCGAATT |
mXYSRa Sal inverse PCR Rv1 | GGCCGTCGACTAAGCCAAATCTTGAAACATAAAG |
