Fig. 2: Knockdown of USP35 enhances ferroptosis invoked by ferroptosis inducer in ER+ breast cancer cells. | Communications Biology

Fig. 2: Knockdown of USP35 enhances ferroptosis invoked by ferroptosis inducer in ER+ breast cancer cells.

From: USP35 promotes the growth of ER positive breast cancer by inhibiting ferroptosis via BRD4-SLC7A11 axis

Fig. 2

A Ferroptosis inducers increased the growth inhibition of cells induced by USP35 knockdown. MCF-7 and ZR-75-1 cells with control-shRNA (sh) and USP35-shRNAs (sh#1, sh#2) were treated with Erastin (10 μM) or RSL3 (5 μM) for 2 d before being subjected to CCK-8 assay (n = 3). B Knockdown of USP35 expression enhanced lipid peroxidation caused by Erastin or RSL3 treatment. MCF-7 and ZR-75-1 cells with control-shRNA and USP35-shRNAs were treated with Erastin (10 μM) or RSL3 (5 μM) and stained with 5 μM C11-BODIPY followed by flow cytometry analysis after 24-h treatment. Quantitation and statistical analysis of the data were shown on the right (n = 3). C Knockdown of USP35 increased MDA concentration induced by RSL3 treatment (n = 3). D USP35 was overexpressed in MCF-7 and ZR-75-1 cells (n = 3). E USP35 overexpression increased cell growth inhibited by RSL3 treatment. MCF-7 and ZR-75-1 cells with USP35 overexpression were treated with RSL3 (5 μM) for 3 d before being subjected to the colony formation assay (n = 3). F USP35 overexpression reduced the elevated level of lipid peroxidation induced by RSL3 (n = 3). All experiments were performed at least three times. Data are shown as Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ###P < 0.001.

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