Fig. 5: HKI-VDAC binding critically relies on an asymmetric positioning of the membrane-buried Glu.
From: Hexokinase-I directly binds to a charged membrane-buried glutamate of mitochondrial VDAC1 and VDAC2
a Stills from MD simulations of VDAC1 and VDAC1E73F/F71E with the membrane-facing Glu and Phe residues at positions 71 and 73 represented as red and white balls, respectively. The graphs show the position of Glu73 in VDAC1 and Glu71 in VDAC1E73F/F71E relative to the membrane center (dashed line) over the course of a simulation. b Relative duration of contacts between HKI-Met1 and specific residues of VDAC1 or VDAC1E73F/F71E with cytosol- or IMS-facing C-termini. Data for VDAC1 are taken from Fig. 3f and shown for comparison. For VDAC1E73F/F71E data of three individual simulations were combined with a total simulation time between 148 µs and 162 µs per condition. c Fluorescence images of VDAC1/2-DKO HeLa cells co-expressing EGFP-tagged HKI (green) and HA-tagged VDAC1 or VDAC1E73F/F71E, fixed and then stained with DAPI (blue) and antibodies against the HA-epitope (red) and Tom20 (magenta). Line scans showing degree of overlap between HKI and Tom20 signals along the path of the arrow shown in the zoom-in. Scale bar, 10 μm.
