Fig. 3: Reactive oxygen species (ROS) burst detection by H2DCFDA staining in hyphae of Magnaporthe oryzae in response to dsRNA treatment.
Three-day-old Mo liquid cultures were incubated with different RNA molecules for 2 min and subsequently stained for CSLM analysis. A Germinated conidia were treated with 500 ng/µL of poly(I:C) as positive stressor or 10 ng/µL of Pmk1-dsRNA and SHP-siRNA. B Dose-response effect of ROS production in germinating conidia after treatment with SHP-dsRNA. Fluorescence detection with AF488 [λexcitation (nm): 492; λemission (nm): 561]. Scale bar equals 50 µm.
