Fig. 4: Imaging the nuclear transfer of the chimeric GFP-MoHog1p in conidia of Magnaporthe oryzae in response to different stressors. | Communications Biology

Fig. 4: Imaging the nuclear transfer of the chimeric GFP-MoHog1p in conidia of Magnaporthe oryzae in response to different stressors.

From: Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Fig. 4

A Conidia (2000/100 µL) in 0.002% (v/v) Tween20 were incubated with 20 ng/µL of the indicated dsRNA. Potassium chloride (0.25 M) was used as positive stressor control, while geneticin was used as negative control. Images were taken with the AF488 laser at 5 and 60 min after treatment. B Conidia were incubated with 20 ng/µL of the indicated siRNA. C Dose-effect analysis of GFP-MoHog1p nuclear accumulation in Mo conidia treated with various amounts of GFP-dsRNA (476 bp). Scale bar equals 10 µm. AF488 [λexcitation (nm): 492; λemission (nm): 561].

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