Fig. 7: Formulation of dsRNA in alginate-chitosan nanoparticles (NPs). | Communications Biology

Fig. 7: Formulation of dsRNA in alginate-chitosan nanoparticles (NPs).

From: Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Fig. 7

A Sketch of the formulation process of dsRNA-alginate-chitosan NPs. dsRNA and alginate are mixed in equal ratio to form an anionic solution. Chitosan is added to this solution, the mixture is vortexed and incubated at room temperature for 1 h. The NPs form through electrostatic interaction of positive amine groups with negative phosphate and carboxyl groups from chitosan, dsRNA, and alginate, respectively. B Particle size distributions for Pmk1-dsRNA-NPs. Samples of Pmk1-dsRNA-alginate-chitosan NPs were characterized by dynamic light scattering (DLS). The peak of each distribution is the median of the distribution. Values are calculated by averaging the results from the three graphs shown in the figure. The normalized intensity (Id), volume (Vd), and number distributions (Nd) of n = 3 samples (■, ●, ▲, respectively) are on a logarithmic diameter scale.

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